Coding

Part:BBa_K2114999:Design

Designed by: Wladislaw Stroukov   Group: iGEM16_Freiburg   (2016-10-05)


pelB_aGFPnano_TEV_10XHis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To ensure the formation of the characteristic disulfide bond of the nanobody the pelB leader sequence was included in the N-terminus of the nanobody by primer extensions and Gibson assembly.


Source

the anti-GFP nanobody was provided by Dr. Maximilian Ulbrich (BIOSS)

References